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Search results for: Text "Gallic acid" - all of the words(Protocol search)

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Pub. Date: Apr-09-2016; DOI:10.1007/978-1-4939-3332-7_5
Summary: (v/v). 6. 50 % Ethanol solution (pH 3.6—v/v). 7. 5 % Sodium carbonate solution (m/v). 8. 1 % Hydrochloric acid solution (v/v). 9. Folin-Ciocalteu phenol reagent. 10. Gallic acid standard. 11...
Pub. Date: Jan-01-2015; DOI:10.1007/978-1-4939-1441-8_16
Summary: to PP concentration that is used in this assay to calculate antioxidant activity with respect to an antioxidant standard, gallic acid (GA), wherein the slope of a color intensity versus concentration...
Pub. Date: Apr-09-2016; DOI:10.1007/978-1-4939-3332-7_16
Summary: . Nylon filter (0.45 μm). 2.3 Estimation of Total Phenols 1. 0.2 N Folin–Ciocalteu (FC) reagent. Store at 4 °C. 2. 1000 ppm gallic acid stock solution. Store at 4 °C in dark condition (see Note...
Pub. Date: Apr-09-2016; DOI:10.1007/978-1-4939-3332-7_9
Summary: °C. 2. 1000 ppm Gallic acid . Store at 4 °C in the dark (see Note 5 ). 3. 20 % sodium carbonate stock solution. Store at 4 °C. 4. 80 % methanol, high pressure liquid chromatography (HPLC ) grade. 2.5...
Pub. Date: Apr-01-2009; DOI:10.1007/978-1-60327-287-2_1
Summary: at 120°C 1.2.2 Estimation of Total Phenols 1. 2 N folin-cicalteu reagent (Sigma). Store at 4°C. 2. Gallic acid (Sigma). Generally 1,000 ppm stock is prepared and stored at 4°C in the dark (see Note 4). 3...
Pub. Date: Apr-09-2016; DOI:10.1007/978-1-4939-3332-7_1
Summary: water. 6. HPLC -grade glacial acetic acid. 7. Stock solutions (1 mg/ml, prepared in acetonitrile:water, 1:1) of standards of gallic acid , syringic acid , p-hydroxybenzoic acid (HBA), and caffeic...
Pub. Date: Sept-02-2015; DOI:10.1007/978-1-4939-2684-8_24
Summary: manufacturer in darkness and use as a neat solution (room temperature). 3. Gallic acid stock solution (30 mM): weigh out 5 mg of gallic acid into a 2 mL eppendorf tube. Add 1 mL of methanol and dissolve...
Pub. Date: Mar-19-2007; DOI:10.1007/978-1-59745-098-0_4
Summary: . Reflux for 2 h, allow to cool, make up to 1 L, and store in dark (30). 3. Saturated sodium carbonate solution. 4. Gallic acid. 5. 1 N NaOH. 6. 2 N HCl. 2.1 cDNA Isolation, Expression, and Enzyme...
Pub. Date: Apr-09-2016; DOI:10.1007/978-1-4939-3332-7_2
Summary: acid (2,4-D ). 2.3 Extraction and Estimation of Total Phenolics 1. Folin-Ciocalteu reagent. 2. Gallic acid (see Notes 1 and 2 ). 3. 20 % (w/v) sodium carbonate solution: Prepare a stock solution...
Pub. Date: July-06-2007; DOI:10.1007/978-1-59745-425-4_13
Summary: tannins but may provide limited information for complex oligomeric hydrolyzable tannins. The rhodanine method (9) determines gallotannins as gallic acid equivalent. In this method, gallotannins...
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