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Search results for: Text "DEAE-Sepharose® Fast Flow" - all of the words(Protocol search)

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Pub. Date: June-01-2001; DOI:10.1385/1-59259-209-0:221
Summary: (e.g., Kontes Flex), each packed with 80-mL DEAE-Sepharose Fast Flow (Pharmacia). 6. A 2.5 cm by 20 cm guard column packed with 30-mL Sepharose-4B (Pharmacia). 2.3 Hydrophobic Interaction...
Pub. Date: Jan-01-2015; DOI:10.1007/978-1-4939-1714-3_2
Summary: : 210 mg Riboflavin, 2.7 g pantothenic acid, 3.0 g niacin, 0.7 g pyridoxin, 30 mg biotin, 20 mg folic acid, q.s. with water to 500 mL, store protected from light. 6. Econo-column. 7. DEAE-Sepharose Resin...
Author(s): Lucy Waskell
Pub. Date: May-29-2003; DOI:10.1385/1-59259-400-X:3
Summary: C, plus 0.4 M NaCl. 11. Buffer E: 20 mM phosphate buffer, pH 8.0, 1 mM EDTA, 0.4% Na deoxycholate. 12. Buffer F: 50 mM Tris-acetate, pH 8.1 at 22°, 1 mM EDTA. 13. Resins: DEAE Sepharose Fast Flow (Sigma...
Pub. Date: Jan-01-2014; DOI:10.1007/978-1-62703-724-2_7
Summary: –HCl, 1 M Na2SO4, pH 7.5. 7. A solution of 50 % polyethylene glycol (PEG) is made from 1 kg PEG 6000 (Merck) and 1 l of water. 8. Resins: The resins DEAE Sepharose fast flow, Sephacryl S-300...
Pub. Date: Nov-02-2010; DOI:10.1007/978-1-60761-913-0_12
Summary: observations), the anion-exchange option is favoured. To illustrate this, Fig. 2 and Table 2 demonstrate how anion-exchange chromatography using DEAE-Sepharose® Fast Flow (GE Healthcare) can be used...
Pub. Date: Sept-15-2000; DOI:10.1385/1-59259-062-4:1
Summary: NaCl, 15% glycerol. 3. Buffer D (200 mL): 20 mM TEA pH 7.7, 1 M NaCl. 4. DEAE-Sepharose Fast Flow anion-exchange column chromatography matrix (Pharmacia), approximately 65 mL. 5. Chromatography...
Pub. Date: Mar-04-2003; DOI:10.1385/1-59259-373-9:031
Summary: Chromatography 2.1.1.1 Purification of Hb on DEAE-Sepharose Fast Flow: Small-Scale Purification (see Notes 1 and 2) 1. XK 16/10 chromatographic: column (Amersham Pharmacia Biotech). 2. DEAE-Sepharose Fast Flow...
Pub. Date: May-01-2009; DOI:10.1007/978-1-59745-558-9_12
Summary: (Sigma-Aldrich, Munich, Germany), and 15N-rUTP (Silantes, Munich, Germany). 2. DEAE-Sepharose column (Amersham Pharmacia Biotech, Uppsala, Sweden). 3. DEAE equilibration solution: 0.1M Na-acetate, pH...
Author(s): Yu Yamaguchi
Pub. Date: June-01-2001; DOI:10.1385/1-59259-209-0:035
Summary: . Apply the combined soluble fraction onto a column of DEAE-Sepharose Fast Flow preequilibrated with buffer C. For 1000 mL extract, a column with 25 to 30-mL bed volume is sufficient. It takes several hours...
Pub. Date: Mar-10-2000; DOI:10.1385/1-59259-052-7:89
Summary: of concentrated protein to remove insoluble material. 2. Load the sample on a DEAE-Sepharose column (DEAE-Sepharose Fast Flow. Pharmacia Biotech, code no. 17-0709-01) that has been equilibrated in 10 mM...
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