Results 1 - 10 of 51
Search results for: subject "Embryology"/ (Protocol search)
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Pub. Date:
July-01-1999;
DOI:10.1385/1-59259-270-8:101
Summary: The introduction and establishment of transgenic, and in particular embryonic stem (ES) cell-based gene “knockout” technologies have made the mouse a key player in studying embryonic development and...
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Pub. Date:
July-01-1999;
DOI:10.1385/1-59259-270-8:123
Summary: Gene trapping in mouse embryonic stem (ES) cells offers a method to create random developmental mutants with a direct route to cloning and defining the expression pattern of the disrupted gene (1)....
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Pub. Date:
July-01-1999;
DOI:10.1385/1-59259-270-8:139
Summary: The purpose of this chapter is to provide some of the rationale leading up to the development of the H-2Kb
tsA58 transgenic mouse, a prototype of animals that can be used to create conditionally...
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Pub. Date:
July-01-1999;
DOI:10.1385/1-59259-270-8:159
Summary: The development of transgenic technology during recent years has allowed researchers to probe much more deeply than was previously possible into the molecular mechanisms influencing embryonic development....
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Pub. Date:
July-01-1999;
DOI:10.1385/1-59259-270-8:183
Summary: Cell lineage analyses trace the hierarchy of cell types derived from a progenitor population. Critical to these analyses is the ability to track reliably all or defined subsets of the clonal descendants...
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Pub. Date:
July-01-1999;
DOI:10.1385/1-59259-270-8:201
Summary: Primordial germ cells (PGCs) in the embryo give rise to functional gametes in the adult animal. Considering their importance in the continuation of the species, it is no wonder that there is much interest...
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Pub. Date:
July-01-1999;
DOI:10.1385/1-59259-270-8:215
Summary: The major advantage of the avian embryo for the embryologist is its accessibility for manipulation and observation. Indeed, it is for this reason that, historically, detailed descriptions of normal...
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Pub. Date:
July-01-1999;
DOI:10.1385/1-59259-270-8:221
Summary: The following methods comprise a brief description of chick egg incubation and embryo harvesting. For further details concerning optimizing embryo viability during incubation, the reader is referred...
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Pub. Date:
July-01-1999;
DOI:10.1385/1-59259-270-8:225
Summary: As the title indicates, this culture method was developed by Denis New and first described in 1955 (1). It enables the observer to study the events of gastrulation in the chick embryo in much greater...
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Pub. Date:
July-01-1999;
DOI:10.1385/1-59259-270-8:23
Summary: Interactions between epithelial and mesenchymal tissues constitute a central mechanism regulating the development of most embryonic organs. Studies on the nature of such interactions require the separation...
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