SpringerProtocols: Abstract: 4. First Analysis of Macromolecular Crystals: Biochemistry and X-Ray Diffraction

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4. First Analysis of Macromolecular Crystals: Biochemistry and X-Ray Diffraction

By: David Jeruzalmi²

Abstract

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Methods are described for performing a first analysis of newly prepared macromolecular crystals. Biochemical analysis using denaturing gel electrophoresis on dissolved crystals informs on the content of the crystal. Detailed protocols are given for mounting crystals in capillaries or fiber loops in preparation for analysis of their X-ray diffractive properties.

Affiliation(s): (2) Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA

Book Title: Macromolecular Crystallography Protocols: Volume 2: Structure Determination

Series: Methods in Molecular Biology | Volume: 364 | Pub. Date: Feb-05-2007 | Page Range: 43-61 | DOI: 10.1385/1-59745-266-1:43

Subject: Protein Science

Key Words: Macromolecular crystals - biochemical analysis of crystal contents - crystal mounting - X-ray diffraction - crystal symmetry - space groups

References

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