Springer Protocols: Abstract: Isolation of the Platelet Releasate

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Isolation of the Platelet Releasate

By: Judith Coppinger³, Desmond J. Fitzgerald⁴, Patricia B. Maguire⁴

Abstract

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This chapter describes an approach to isolate, separate, and identify the contents of the platelet releasate, a fraction highly enriched for platelet granular and exosomal contents. Investigation into such a fraction will improve our understanding of platelet interactions with other cells, vascular remodeling, coagulation, and vessel growth.

Affiliation(s): (3) Department of Clinical Pharmacology, Royal College of Surgeon’s in Ireland, Dublin, Ireland
(4) Department of Molecular Medicine, Conway Institute, University College Dublin, Dublin, Ireland

Book Title: Cardiovascular Proteomics: Methods and Protocols

Series: Methods in Molecular Biology | Volume: 357 | Pub. Date: Feb-05-2007 | Page Range: 307-311 | DOI: 10.1385/1-59745-214-9:307

Subject: Molecular Medicine

Key Words: Platelet - secretion - releasate - proteomics

References

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6.	Coppinger, J. A., Cagney, G., Toomey, S., et al. (2004) Characterization of the proteins released from activated platelets leads to localization of novel platelet proteins in human atherosclerotic lesions. Blood 103, 2096–2104.

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