Springer Protocols: Abstract: 4. Construction of Mouse Adenovirus Type 1 Mutants

4. Construction of Mouse Adenovirus Type 1 Mutants

By: Angela N. Cauthen², Amanda R. Welton³, Katherine R. Spindler³

Abstract

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Mouse adenovirus provides a model for studying adenovirus pathogenesis in the natural host. The ability to make viral mutants allows the investigation of specific mouse adenoviral gene contributions to virus-host interactions. Methods for propagation and titration of wild-type mouse adenovirus, production of viral DNA and viral DNA-protein complex, and transfection of mouse cells to obtain mouse adenovirus mutants are described in this chapter. Plaque purification, propagation, and titration of the mutant viruses are also presented.

Affiliation(s): (2) Department of Natural Sciences, Clayton College and State University, Morrow, GA
(3) Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI

Book Title: Adenovirus Methods and Protocols: Volume 1: Adenoviruses, Ad Vectors, Quantitation, and Animal Models

Series: Methods in Molecular Medicine | Volume: 130 | Pub. Date: Feb-27-2007 | Page Range: 41-59 | DOI: 10.1385/1-59745-166-5:41

Subject: Microbiology

Key Words: Mouse adenovirus type 1 - virus purification - transfection - plaque assay - DNA-protein complex - virus mutants

References

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