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2. Isolation, Growth, and Purification of Defective Adenovirus Deletion Mutants
Abstract
Defective adenovirus deletion mutants can be grown by complementation in the presence of helper viruses that supply essential functions missing in the deletion mutant. In general, the deletion mutant then must be separated physically from the helper for use in subsequent experiments. This chapter includes suggestions for selection of helper viruses, protocols for the production of stocks by complementation, and procedures for physical separation of deletion mutants from their helpers.
Affiliation(s): (2) Department of Molecular Microbiology and Immunology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD
(3) Department of Genetic Medicine, Weill Medical College of Cornell University, New York, NY
Series: Methods in Molecular Medicine  |  Volume: 130  |  Pub. Date: Feb-27-2007  |  Page Range: 19-28  |  DOI: 10.1385/1-59745-166-5:19
Subject:  Microbiology
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