SpringerProtocols: Abstract: 1. Manipulation of Early Region 4

1. Manipulation of Early Region 4

By: Julie Boyer², Gary Ketner³

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Adenovirus early region 4 (E4) regulates processes in infected cells that include viral late gene expression, nonhomologous end joining, responses to DNA damage, and apoptosis. E4 is essential for viral growth in most cell lines. In this chapter, the current knowledge of the functions of six E4 products is summarized briefly. Protocols are presented for manipulation of E4, incorporation of E4 mutations into the viral genome, and growth of E4 mutants on complementing cell lines. A compilation of the described E4-complementing cell lines is included.

Affiliation(s): (2) Department of Genetic Medicine, Weill Medical College of Cornell University, New York, NY
(3) Department of Molecular Microbiology and Immunology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD

Book Title: Adenovirus Methods and Protocols: Volume 1: Adenoviruses, Ad Vectors, Quantitation, and Animal Models

Series: Methods in Molecular Medicine | Volume: 130 | Pub. Date: Feb-27-2007 | Page Range: 1-17 | DOI: 10.1385/1-59745-166-5:1

Subject: Microbiology

Key Words: Complementing cell line - DNA-protein complex - overlap recombination - ligation - bacterial recombination - mutant screening - adenovirus - early region 4 - mutant - mutant construction - complementation

References

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