SpringerProtocols: Abstract: 6. Discovery of Differentially Expressed Genes: Technical Considerations

6. Discovery of Differentially Expressed Genes: Technical Considerations

By: Øystein Røsok², Mouldy Sioud²

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Identification and characterization of differentially expressed genes may be an important first step toward the understanding of both normal physiology and disease. A multitude of techniques belonging to two main categories have been developed to identify the differences in gene expression between samples from different biological origin: selection techniques and global techniques. Whereas the selection techniques strive to identify specific differentially expressed genes, the global techniques analyze the total transcriptome or a major part of the RNA population in a defined biological material. By exploiting the known sequences of the adaptors used in suppressive subtraction hybridization technique, a strategy named novel rescue—suppression-subtractive hybridization was developed. It should facilitate the discovery of differentially expressed genes.

Affiliation(s): (2) Department of Immunology, Institute for Cancer Research, The Norwegian Radium Hospital, University of Oslo, Oslo, Norway

Book Title: Target Discovery and Validation Reviews and Protocols: Volume 1, Emerging Strategies for Targets and Biomarker Discovery

Series: Methods in Molecular Biology | Volume: 360 | Pub. Date: Oct-15-2006 | Page Range: 115-129 | DOI: 10.1385/1-59745-165-7:115

Subject: Pharmacology/Toxicology

Key Words: Differential display - microarray - representational difference analysis (RDA) - serial analysis of gene expression (SAGE) - target discovery - target identification

References

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