SpringerProtocols: Abstract: 9. Selective Isolation of Large Chromosomal Regions by Transformation-Associated Recombination Cloning for Structural and Functional Analysis of Mammalian Genomes

9. Selective Isolation of Large Chromosomal Regions by Transformation-Associated Recombination Cloning for Structural and Functional Analysis of Mammalian Genomes

By: Natalay Kouprina², Vladimir N. Noskov², Vladimir Larionov²

Abstract

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Transformation-associated recombination (TAR) cloning allows selective isolation of full-size genes and genomic loci as circular yeast artificial chromosomes in yeast. The method has a broad application for structural and functional genomics, long-range haplotyping, characterization of chromosomal rearrangements, and evolutionary studies. This chapter describes a basic protocol of gene isolation by TAR, as well as a method of conversion of TAR isolates into bacterial artificial chromosomes.

Affiliation(s): (2) Laboratory of Biosystems and Cancer, National Cancer Institute (NCI, NIH), Bethesda, MD

Book Title: YAC Protocols

Series: Methods in Molecular Biology | Volume: 349 | Pub. Date: Aug-18-2006 | Page Range: 85-101 | DOI: 10.1385/1-59745-158-4:85

Subject: Genetics/Genomics

Key Words: YAC - TAR cloning - gene isolation - complex genomes

References

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