SpringerProtocols: Abstract: 8. Construction of Plasmids for RNA Interference and In Vitro Transcription of Double-Stranded RNA

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8. Construction of Plasmids for RNA Interference and In Vitro Transcription of Double-Stranded RNA

By: Lisa Timmons⁴

Abstract

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Double-stranded RNA (dsRNA)-induced gene silencing in Caenorhabditis elegans involves the manufacture and delivery of defined sequences of dsRNA to the organism, followed by a careful monitoring for loss-of-function phenocopies in treated animals. In this chapter, we describe how to generate DNAs that can be used as templates for transcription of dsRNA.

Affiliation(s): (4) Department of Molecular Biosciences, University of Kansas, Lawrence, KS

Book Title: C. elegans: Methods and Applications

Series: Methods in Molecular Biology | Volume: 351 | Pub. Date: Aug-15-2006 | Page Range: 109-117 | DOI: 10.1385/1-59745-151-7:109

Subject: Genetics/Genomics

Key Words: Double-stranded RNA - dsRNA - single-stranded RNA - ssRNA - RNAi - RNA silencing - feeding - soaking - injection - HT115 - HT115(DE3) - transgene - hairpin RNA - RNaseIII - transcription - functional genomics

References

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