SpringerProtocols: Abstract: Rose (Rosa hybrida L.)

Rose (Rosa hybrida L.)

By: Schuyler S. Korban³, Ksenija Gasic³, Xiangqian Li⁴

Abstract

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Although rose transformation is successful, it remains difficult to transform myriad rose species as well as different rose genotypes. In this protocol, a detailed description of rose transformation is presented. This protocol relied on Agrobacterium-mediated transfer of embryogenic callus cultures. There are many critical steps that must be followed to achieve successful transformation; however, it is important to keep in mind that these apply to a selected number of genotypes, and as a different genotype is subjected to transformation, modifications of this protocol must be made to achieve successful transformation.

Affiliation(s): (3) Department of Natural Resources & Environmental Sciences, University of Illinois, 310 ERML, Urbana, IL
(4) Division of Biological Sciences, University of California-San Diego, La Jolla, CA

Book Title: Agrobacterium Protocols Volume 2

Series: Methods in Molecular Biology | Volume: 344 | Pub. Date: Sep-01-2006 | Page Range: 351-358 | DOI: 10.1385/1-59745-131-2:351

Subject: Plant Sciences

Key Words: Agrobacterium tumefaciens - Rosa hybrida - genetic transformation

References

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