Springer Protocols: Abstract: Grapevine (Vitis vinifera L.)

Grapevine (Vitis vinifera L.)

By: Alain Bouquet³, Laurent Torregrosa³, Pat Iocco⁴, Mark R. Thomas⁴

Abstract

Full Text

Download PDF (520K)

Grapevine (Vitis) is considered to be one of the major fruit crops in the world based on hectares cultivated and economic value. Grapes are used not only for wine but also for fresh fruit, dried fruit, and juice production. Wine is by far the major product of grapes and the focus of this chapter is on wine grape cultivars. Grapevine cultivars of Vitis vinifera L. have a reputation for producing premium quality wines. These premium quality wines are produced from a small number of cultivars that enjoy a high level of consumer acceptance and are firmly entrenched in the market place because of varietal name branding and the association of certain wine styles and regions with specific cultivars. In light of this situation, grapevine improvement by a transgenic approach is attractive when compared to a classical breeding approach. The transfer of individual traits as single genes with a minimum disruption to the original genome would leave the traditional characteristics of the cultivar intact. However, a reliable transformation system is required for a successful transgenic approach to grapevine improvement. There are three criteria for achieving an efficient Agrobacterium-mediated transformation system: (1) the production of highly regenerative transformable tissue, (2) optimal co-cultivation conditions for both grapevine tissue and Agrobacterium, and (3) an efficient selection regime for transgenic plant regeneration. In this chapter, we describe a grapevine transformation system which meets the above mentioned criteria.

Affiliation(s): (3) UMR Biology of Development of Cultivated Perennial Plants, Agro-M/INRA, 2, place Pierre Viala, 34060 Montpellier Cedex1, France
(4) CSIRO Plant Industry, Horticulture Unit, PO Box 350, Glen Osmond, SA, 5064, Australia

Book Title: Agrobacterium Protocols Volume 2

Series: Methods in Molecular Biology | Volume: 344 | Pub. Date: Sep-01-2006 | Page Range: 273-285 | DOI: 10.1385/1-59745-131-2:273

Subject: Plant Sciences

Key Words: Grapevine - Vitis - embryogenic callus - Agrobacterium - selectable markers - transgenic - antibiotic sensitivity - reporter genes - plant regeneration - transformation efficiency

References

Download References

1.	Mullins, M.G., Bouquet, A., and Williams, L.E. (1992) Biology of Grapevine. Cambridge University Press, Cambridge, UK.

2.	Kikkert, J.R., Thomas, M.R., and Reisch, B.I. (2001) Grapevine genetic engineering. In: Molecular Biology and Biotechnology of the Grapevine (Roubelakis-Angelakis, K.A., ed.), Kluwer Academic Publishers, Dordrecht, The Netherlands, pp. 393–410.

3.	Bouquet, A., Chatelet, P., and Torregrosa, L. (2003) Grapevine genetic engineering: tool for genome analysis or plant breeding method? Which future for transgenic vines? AgBiotechNet 5, ABN 116, 1–10.

4.	Colova-Tsolova, V., Perl, A., Krastanova, S., Tsvetkov, I., and Atanassov, A. (2001) Genetically engineered grape for disease and stress tolerance. In: Molecular Biology and Biotechnology of the Grapevine (Roubelakis-Angelakis, K.A., ed.), Kluwer Academic Publishers, Dordrecht, The Netherlands, pp. 411–432.

5.	Mullins, M.G., Tang, F.C.A., and Facciotti, D. (1990) Agrobacterium-mediated genetic transformation of grapevines. Transgenic plants of Vitis rupestris Scheele and buds of Vitis vinifera L. Nat. Biotechnol. 8, 1041–1045.

6.	Perl, A., Colova-Tsolova, V., and Eshdat, Y. (2004) Agrobacterium-mediated transformation of grape embryogenic calli. In: Transgenic Crops of the World (Curtis, I.S., ed.), Kluwer Academic Publishers, Dordrecht, The Netherlands, pp. 1–14.

7.	Colby, S.M., Juncosa, A.M., and Meredith, C.P. (1991) Cellular differences in Agrobacterium susceptibility and regenerative capacity restrict the development of transgenic grapevines. J. Amer. Soc. Hort. Sci. 116, 356–361.

8.	Mezzetti, B., Pandolfini, T., Navacchi, O., and Landi, L. (2002) Genetic transformation of Vitis vinifera via organogenesis. BMC Biotechnology 2, 18–28.

9.	Martinelli, L. and Gribaudo, I. (2001) Somatic embryogenesis in grapevine. In: Molecular Biology and Biotechnology of the Grapevine (Roubelakis-Angelakis, K.A., ed.), Kluwer Academic Publishers, Dordrecht, The Netherlands, pp. 327–351.

10.	Le Gall, O., Torregrosa, L., Danglot, Y., Candresse, T., and Bouquet, A. (1994) Agrobacterium-mediated genetic transformation of grapevine somatic embryos and regeneration of transgenic plants expressing the coat protein of grapevine chrome mosaic nepovirus (GCMV). Plant Science 102, 161–170. <Occurrence Type="DOI"><Handle>10.1016/0168-9452(94)90034-5</Handle></Occurrence>

11.	Perl, A., Lotan, O., Abu-Abied, M., and Holland, D. (1996) Establishment of an Agrobacterium-mediated transformation system for grape (Vitis vinifera L.): The role of antioxidants during grape-Agrobacterium interactions. Nat. Biotechnol. 14, 624–628.

12.	Franks, T., Gang He, D., and Thomas, M., (1998) Regeneration of transgenic Vitis vinifera L. Sultana plants: genotypic and phenotypic analysis. Mol. Breeding 4, 321–333.

13.	Reustle, G.M., Wallbraun, M., Zwiebel, M., et al. (2002) Experience with different selectable marker systems for the genetic engineering of grapevine. Acta Hort.. 603, 485–490.

14.	Torregrosa, L., Péros, J.-P., Lopez, G., and Bouquet, A. (2000) Effect of hygromycin, kanamycin and phosphinothricin on the embryogenic development and axillary micropropagation of Vitis vinifera L. Acta Hort. 528, 401–406.

15.	Kieffer, F., Triouleyre, C., Bertsch, C., Farine, S., Leva, Y., and Walter, B. (2004) Mannose and xylose cannot be used as selectable agents for Vitis vinifera L. Vitis 43, 35–39.

16.	Hood, E.E., Gelvin, S.B., Melchers, L.S., and Hoekema, A. (1993) New Agrobacterium helper plasmids for gene transfer to plants. Transgenic Res. 2, 208–218.

17.	Torregrosa, L., Iocco, P., and Thomas, M.R. (2002) Influence of Agrobacterium strain, culture medium, and cultivar on the transformation efficiency of Vitis vinifera L. Am. J. Enol. Vitic. 53, 183–190.

18.	Garfinkel, D.J. and Nester, E.W. (1980) Agrobacterium mutants affected in crown gall tumerorigenesis and octopine catabolism. J. Bacteriol. 144, 732–743.

19.	Chilton, M.D., Currier, T.C., Farrand, S.K., Bendish, A.J., Gordon, M.P., and Nester, E.W. (1974) Agrobacterium tumefaciens DNA and PS8 bacteriophage DNA not detected in crown gall tumors. Proc. Natl. Acad. Sci. USA 71, 3672–3676.

20.	Nitsch, J.-P. and Nitsch, C. (1969) Haploid plants from pollen grains. Science 165, 85–87.

21.	Gamborg, O.L., Miller, R.A., and Ojima, K. (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp. Cell Res. 50, 1515–1518. <Occurrence Type="DOI"><Handle>10.1016/0014-4827(68)90403-5</Handle></Occurrence>

22.	Murashige, T. and Skoog, F. (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15, 473–497.

23.	Torregrosa, L., Torres-Viñals, M., and Bouquet, A. (1995) Somatic embryogenesis from leaves of Vitis x Muscadinia hybrids. Vitis 34, 239–240.

24.	Torregrosa, L. (1998) A simple and efficient method to obtain stable embryogenic cultures from anthers of Vitis vinifera L. Vitis 37, 91–92.

25.	Galzy, R., Haffner, V., and Compan, D. (1990) Influence of three factors on the growth and nutrition of grapevine microcuttings. J. Exp. Bot. 41, 295–301.

26.	Iocco, P., Franks, T., and Thomas, M.R. (2001) Genetic transformation of major wine grape cultivars of Vitis vinifera L. Transgenic Res. 10, 105–112.

27.	Bowers, J., Boursiquot, J.-M., This, P., Chu, K., Johansson, H., and Meredith, C. (1999) Historical genetics: The parentage of Chardonnay, Gamay and other wine grapes of Northeastern France. Science 285, 1562–1565.

28.	Mullins, M.G. (1966) Test-plants for investigations of physiology of fruiting in Vitis vinifera L. Nature 209, 419–420.

29.	Martinelli, L. and Mandolino, G. (2001) Transgenic grapes (Vitis species). In: Biotechnology in Agriculture and Forestry, Vol.47, Transgenic Crops II (Bajaj, Y.P.S., ed.), Springer-Verlag, Berlin, Germany, pp. 325–338.

30.	Legrand, V., Dalmayrac, S., Latché, A., et al. (2003) Constitutive expression of Vr-ERE gene in transformed grapevines confers enhanced resistance to eutypine, a toxin from Eutypa lata. Plant Science 164, 809–814. <Occurrence Type="DOI"><Handle>10.1016/S0168-9452(03)00069-4</Handle></Occurrence>

31.	Motoike, S.Y., Skirvin, R.M., Norton, M.A., and Otterbacher, A.G. (2002) Development of methods to genetically transform American grape (Vitis × labruscana L.H. Bailey). J. Hort. Sci. Biotech. 77, 691–696.

32.	Mauro, M.-C., Toutain, S., Walter, B., et al. (1995) High efficiency regeneration of grapevine plants transformed with the GFLV coat protein gene. Plant Science 112, 97–106. <Occurrence Type="DOI"><Handle>10.1016/0168-9452(95)04246-Q</Handle></Occurrence>

33.	Bouquet, A. and Torregrosa, L. (2003) Micropropagation of the grapevine. In: Micropropagation of Woody Trees and Fruits (Jain, S.M. and Ishii, K., eds.), Kluwer Academic Publishers, Dordrecht, The Netherlands, pp. 319–352.

34.	Torregrosa, L., Bouquet, A., and Goussard, P.G. (2001) In vitro culture and propagation of grapevine. In: Molecular Biology and Biotechnology of the Grapevine (Roubelakis-Angelakis, K.A., eds.), Kluwer Academic Publishers, Dordrecht, The Netherlands, pp. 281–326.

Comments (Loading...)

Post comment/View all comments