Springer Protocols: Abstract: Blueberry (Vaccinium corymbosum L.)

Blueberry (Vaccinium corymbosum L.)

By: Guo-Qing Song³, Kenneth C. Sink³

Abstract

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Recent advances in plant biotechnology have led to a reliable and reproductive method for genetic transformation of blueberry. These efforts built on previous attempts at transient and stable transformation of blueberry that demonstrated the potential of Agrobacterium tumefaciens-mediated transformation, and as well, the difficulties of selecting and regenerating transgenic plants. As a prerequisite for successful stable transformation, efficient regeneration systems were required despite many reports on factors controlling shoot regeneration from leaf explants. The A. tumefaciens-mediated transformation protocol described in this chapter is based on combining efficient regeneration methods and the results of A. tumefaciens-mediated transient transformation studies to optimize selected parameters for gene transfer. The protocol has led to successful regeneration of transgenic plants of four commercially important highbush blueberry cultivars.

Affiliation(s): (3) Plant Transformation Center, Department of Horticulture, Michigan State University, East Lansing, MI

Book Title: Agrobacterium Protocols Volume 2

Series: Methods in Molecular Biology | Volume: 344 | Pub. Date: Sep-01-2006 | Page Range: 263-272 | DOI: 10.1385/1-59745-131-2:263

Subject: Plant Sciences

Key Words: GUS - regeneration - transformation - transgenic plant - transient expression - woody plant

References

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