SpringerProtocols: Abstract: Coffee (Coffea sp.)

Coffee (Coffea sp.)

By: Thierry Leroy³, Edgardo Alpizar³, Magali Dufour³, Hervé Etienne³

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Coffee (Coffea sp.) is a perennial plant widely cultivated in many tropical countries. It is a cash crop for millions of small farmers in these areas. As compared with other tree species, coffee has long breeding cycles that make conventional breeding programs time consuming. For that matter, genetic transformation can be an effective technique to introduce a desired trait in an already “elite” variety, or to study a gene function and expression. In this chapter, we describe two Agrobacterium-mediated transformation techniques; the first with A. tumefaciens to introduce an insect resistance gene and the second with A. rhizogenes to study candidate gene expression for nematode resistance in transformed roots.

Affiliation(s): (3) Centre de Coopération Internationale en Recherche Agronomique pour le Développment, Départment des Cultures Pérennes (CIRAD-CP), TA 80/03, 34398 Montpellier Cedex 5, France

Book Title: Agrobacterium Protocols Volume 2

Series: Methods in Molecular Biology | Volume: 344 | Pub. Date: Sep-01-2006 | Page Range: 191-208 | DOI: 10.1385/1-59745-131-2:191

Subject: Plant Sciences

Key Words: Agrobacterium tumefaciens - Agrobacterium rhizogenes - insect resistance - nematode resistance - Bacillus thuringensis - Meloidogyne sp. - functional genomics

References

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