SpringerProtocols: Abstract: Cassava (Manihot esculenta Crantz)

Cassava (Manihot esculenta Crantz)

By: Weston Msikita³, Uzoma Ihemere⁴, Dimuth Siritunga⁵, Richard T. Sayre⁴

Abstract

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During the last three years the generation of stably transformed cassava plants having value-added traits has become a reality. Currently, two Agrobacterium-mediated transformation systems are routinely used to engineer cassava. These systems use either somatic embryos or friable embryogenic calli. This paper presents detailed protocols for the transformation of cassava using primary somatic embryos. The effects of explant types, tissue culture conditions, and bacterial and plasmid related factors on transformation efficiency are discussed.

Affiliation(s): (3) Department of Molecular Genetics and Cell Biology, University of Chicago, Chicago, IL
(4) Department of Plant Cellular and Molecular Biology, The Ohio State University, Columbus, OH
(5) Department of Biology, University of Puerto Rico, Mayaguez, PR 00681, Puerto Rico

Book Title: Agrobacterium Protocols Volume 2

Series: Methods in Molecular Biology | Volume: 344 | Pub. Date: Sep-01-2006 | Page Range: 13-24 | DOI: 10.1385/1-59745-131-2:13

Subject: Plant Sciences

Key Words: Agrobacterium tumefaciens - apical leaves - auxiliary buds - cassava - direct embryogenesis - explant - friable embryogenic callus - genetic transformation - germinating somatic embryos - Manihot esculenta Crantz - shoot regeneration - somaclonal variation

References

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1.	Nassar, N.M.A., Santos, E., and David, S. (2000) The transference of apomixis genes from Manihot neusana Nassar to cassava, M. esculenta Crantz. Hereditas 132, 167–170.

2.	Nassar, N.M.A. (1995) Development and selection for apomixis in cassava Manihot esculenta Crantz. Can. J. Plant Sci. 74, 857–858.

3.	Hahn, S.K., Bai, K.V., and Asiedu, R. (1990) Tetraploids, triploids and 2n pollen from diploid interspecific crosses with cassava. Theor. Appl. Genet. 79, 433–439.

4.	Nassar, N.M.A. (2003) Gene flow between cassava, Manihot esculenta Crantz, and wild relatives. Genet. Mol. Res. 2, 334–347.

5.	Munyikwa, T.R.I., Raemakers, C.C.J.M., Schreuder, M., et al. (1998) Pinpointing towards improved transformation and regeneration of cassava (Manihot esculenta Crantz). Plant Sci. 135, 87–101. <Occurrence Type="DOI"><Handle>10.1016/S0168-9452(98)00074-0</Handle></Occurrence>

6.	Puonti-Kaerlas, J. (1998) Cassava biotechnology. Biotechnol. Genet. Revs. 15, 329–364.

7.	Taylor, N., Chavarraiga, P., Raemakers, C.C.J.M., Siritunga, D., and Zhang, P. (2004) Development and application of transgenic technologies in cassava. Plant Mol. Biol. 56, 671–688.

8.	Zhang, P., Legris, G., Coulin, P., and Puonti-Kaerlas, J. (2000) Production of stably transformed cassava plants via particle bombardment. Plant Cell Rep. 19, 939–945.

9.	Siritunga, D. and Sayre, R. (2003) Generation of cyanogen-free transgenic cassava. Planta 217, 367–373.

10.	Siritunga, D., Arias-Garzon, D., White, W., and Sayre, R. (2004) Over-expression of hydroxynitrile lyase in transgenic cassava (Manihot esculenta, Crantz) roots accelerates cyanogenesis. Plant Biotech. J. 2, 37–43.

11.	Siritunga, D. and Sayre, R. (2004) Engineering cyanogens in cassava. Plant Mol. Biol. 56, 661–669.

12.	Franche, C., Bogusz, D., Schöpke, C., Fauquet, C., and Beachy, R.N. (1991) Transient gene expression in cassava using high-velocity microprojectiles. Plant Mol. Biol. 17, 493–498.

13.	Zhang, P., Phansiri, S., and Puonti-Kaerlas, J. (2001) Improvement of cassava shoot organogenesis by use of silver nitrate in vitro. Plant Cell Tiss. Org. Cult. 67, 47–54.

14.	Zhang, P. and Puonti-Kaerlas, J. (2000) PIG-mediated cassava transformation using positive and negative selection. Plant Cell Rep. 19, 1041–1048.

15.	Puonti-Kaerlas, J., Li, H.Q., Sautter, C., and Potrykus, I. (1997) Production of transgenic cassava (Manihot esculenta Crantz) via organogenesis and Agrobacterium-mediated transformation. African J. Root Tuber Crops 2, 181–186.

16.	Chavarriaga-Aguirre, P., Schöpke, C., Sangare, A., Fauquet, C.M., and Beachy, R.N. (1993) Transformation of cassava (Manihot esculenta Crantz) embryogenic tissues using Agrobacterium tumefaciens. In: Proceedings of the 1st International Scientific Meeting of the Cassava Biotechnology Network. Cartagena, Colombia, Roca, W.M., Roca and Thro, A.M. eds., CIAT Working Document 123, 222–228.

17.	Msikita, W., Sayre, R.T., White, V.L., and Marks, J. (2002) Influence of explant source, and light on efficiency of Agrobacterium-mediated transformation of cassava. Fifth International Meeting of the Cassava Biotechnology Network. St. Louis, MO., USA. November 4–9.

18.	Konan N.K., Schöpke, C., Carcamo, R., Beachy, R.N., and Fauquet, C. (1997) An efficient mass propagation system for cassava (Manihot esculenta Crantz) based on nodal explants and auxiliary bud-derived meristems. Plant Cell Rep. 16, 444–449.

19.	Puonti-Kaerlas, J., Frey, P., and Potrykus, I. (1997) Development of a meristem gene transfer technique for cassava. African J. Root & Tuber Crops 2, 175–180.

20.	Woodward, B. and Puonti-Kaerlas. J. (2001) Somatic embryogenesis from floral tissue of cassava (Manihot esculenta Crantz). Euphytica 120, 1–6.

21.	Gresshoff, P. and Doy, C. (1974) Derivation of a haploid cell line from Vitis vinifera and the importance of the stage of meiotic development of anthers for haploid culture of this and other genera. Z. Pflanzenphysiol. 73, 132–141.

22.	Raemakers, K., Schreuder, M., Pereira I., Munyikwa, T., Jacobsen, E., and Visser, R. (2001) Progress made in FEC transformation of cassava. Euphytica 120, 15–24.

23.	Taylor, N., Masona, M.V., Carcamo, R., Ho, T., Schöpke, C., and Fauquet, C.M (2001) Production of embryogenic tissues and regeneration of transgenic plants in cassava (Manihot esculenta Crantz). Euphytica 120, 25–34.

24.	Murashige, T. and Skoog, F. (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant. 15, 473–497.

25.	Gonzalez, A.E., Schöpke, C., Taylor, N.J., Beachy, R.N., and Fauquet, C.M. (1998). Regeneration of transgenic cassava plants (Manihot esculenta Crantz) through Agrobacterium-mediated transformation of embryogenic suspension cultures. Plant Cell Reports 17, 827–831.

26.	Reed J., Privalle L., Luann Powell M., et al. (2001) Phosphomannose isomerase: an efficient selectable marker for plant transformation. In Vitro Cell. Dev. Biol. Plant 37, 127–136.

27.	Zambre, M., Terryn, N., De Clercq, J., et al. (2003) Light strongly promotes gene transfer from Agrobacterium tumefaciens to plant cells. Planta 216, 580–586.

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