SpringerProtocols: Abstract: Cork Oak Trees (Quercus suber L.)

Cork Oak Trees (Quercus suber L.)

By: Rubén Álvarez³, Mariano Toribio⁴, Millán Cortizo³, Ricardo-Javier Ordás Fernández³

Abstract

Full Text

Download PDF (544K)

A transformation system for selected mature Quercus suber L. trees using Agrobacterium tumefaciens has been established. Embryos obtained from recurrent proliferating embryogenic masses are inoculated with AGL1 strain harbouring the plasmid pBINUbiGUSint, which carries the nptII and uidA genes. Evidence of stable transgene integration is obtained by polymerase chain reaction for nptII and uidA genes, Southern blotting and expression of the uidA gene. The transgenic embryos are germinated and successfully transferred to soil.

Affiliation(s): (3) Departamento de Biología de Organismos y Sistemas, Universidad de Oviedo, C/. Catedrático Rodrigo Uría Sn, Oviedo, Spain
(4) Instituto Madrileño de Investigación y Desarrollo Rural Agrario y Alimentario (IMIDRA), Finca El “Encín,” Aptdo. 127, Alcalá de Henares, 28800 Madrid, Spain

Book Title: Agrobacterium Protocols Volume 2

Series: Methods in Molecular Biology | Volume: 344 | Pub. Date: Sep-01-2006 | Page Range: 113-123 | DOI: 10.1385/1-59745-131-2:113

Subject: Plant Sciences

Key Words: AGL1 - Agrobacterium tumefaciens - β-glucuronidase - cork oak - Fagaceae - kanamycin resistance - pBINUbiGUSint - Quercus suber - somatic embryogenesis - tree genetic transformation

References

Download References

1.	Álvarez, R., Alonso, P., Cortizo, M., et al. (2004) Genetic transformation of selected mature cork oak (Quercus suber L.) trees. Plant Cell Rep. 23, 218–223.

2.	Sánchez, N., Manzanera, A., Pintos, B., and Bueno, M.A. (2004) Agrobacterium-mediated transformation of cork oak (Quers suber L.). New Forest 29, 169–176.

3.	Carraway, D.T., Wilde, H.D., and Merkle, S.A. (1994) Somatic embryogenesis and gene transfer in American chestnut. J. Am. Chestnut Found. 8, 29–33.

4.	Corredoira, E., Montenegro, D., San-José, M.C., Vieitez, A.M., and Ballester, A. (2004) Agrobacterium-mediated transformation of European chestnut embryogenic cultures. Plant Cell Rep. 23, 311–318.

5.	Rinallo, C. and Mariotti, D. (1993) Rooting of Castanea sativa Mill. shoots: Effect of Agrobacterium rhizogenes T-DNA genes. J. Hort. Sci. 68, 399–407.

6.	Seabra, R.C. and Pais, M.S. (1998) Genetic transformation of European chestnut. Plant Cell Rep. 17, 177–182.

7.	Seabra, R.C. and Pais, M.S. (1999) Genetic transformation of European chestnut (Castanea sativa Mill.) with genes of interest. Acta Hort. 494, 407–414.

8.	Caro, L.A., Santecchia, N., Marinangeli, P.A., Curvetto, N.R., and Hernández, L.F. (2003) Agrobacterium rhizogenes vs auxinic induction for in vitro rhizogenesis of Prosopis chilensis and Nothofagus alpina. Biocell 27, 311–318.

9.	Roest, S., Brueren, H.G.M.J., Evers, P.W., and Vermeer, E. (1991) Agrobacterium-mediated transformation of oak (Quercus robur L.). Acta Hort. 289, 259–260.

10.	Wilhelm, E., Burg, A., Berenyi, M., Endemann, M., and Rodler, R. (1996) Plantlet regeneration via somatic embryogenesis and investigations on Agrobacterium tumefaciens mediated transformation of oak (Quercus robur). In: Somatic cell genetics and molecular genetics of trees (Ahuja, M.R., Boerjan, W., Neale, D.B., eds), Kluwer Academic Publishers, Dordrecht, Netherlands, pp. 119–124.

11.	Merkle, S.A. and Dean, J.F.D. (2000) Forest tree biotechnology. Curr. Opin. Biotech. 11, 298–302. <Occurrence Type="DOI"><Handle>10.1016/S0958-1669(00)00099-9</Handle></Occurrence>

12.	Peña, L. and Séguin, A. (2001) Recent advances in the genetic transformation of trees. Trends Biotechnol. 19, 500–506. <Occurrence Type="DOI"><Handle>10.1016/S0167-7799(01)01815-7</Handle></Occurrence>

13.	Bueno, M.A., Astorga, R., and Manzanera, J.A. (1992) Plant regeneration through somatic embryogenesis in Quercus suber. Physiol. Plant. 85, 30–34.

14.	Manzanera, J.A., Astorga, R., and Bueno, M.A. (1993) Somatic embryo induction and germination in Quercus suber L. Silvae Genet. 42, 90–93.

15.	Fernández-Guijarro, B., Celestino, C., and Toribio, M. (1994) Somatic embryogenesis in Quercus suber L. In: Biotechnology of Trees (Pardos, J.A., Ahuja, M.R., Elena-Rossello, R., eds.), Investigación Agraria, Sistemas y Recursos Forestales no 4, pp. 105–110.

16.	Fernández-Guijarro, B., Celestino, C., and Toribio, M. (1995) Influence of external factors on secondary embryogenesis and germination in somatic embryos from leaves of Quercus suber L. Plant Cell Tissue. Org. Cult. 41, 99–06.

17.	Zobel, B.J. and Talbert, J.T. (1984) Vegetative propagation. In: Applied Forest Tree Improvement, John Wiley and Sons, New York, pp. 309–344.

18.	Hernández, I., Celestino, C., Martínez, I., et al. (2001) Cloning mature cork oak (Quercus suber L.) trees by somatic embryogenesis. Melhoramento 37, 50–57.

19.	Hernández, I., Celestino, C., and Toribio, M. (2003) Vegetative propagation of Quercus suber L. by somatic embryogenesis. I. Factors affecting the induction in leaves from mature cork oak trees. Plant Cell Rep. 21, 759–764.

20.	Hernández, I., Celestino, C., Alegre, J., and Toribio, M. (2003) Vegetative propagation of Quercus suber L. by somatic embryogenesis. II. Plant regeneration from selected cork oak trees. Plant Cell Rep. 21, 765–770.

21.	Lazo, G.R., Stein, P.A., and Ludwig, R.A. (1991) A DNA transformation-competent Arabidopsis genomic library in Agrobacterium. Nat. Biotechnol. 9, 963–967.

22.	Humara, J., Martín, M.S., Parra, F., and Ordás, R.J. (1999) Improved efficiency of uidA gene transfer in stone pine (Pinus pinea) cotyledons using a modified binary vector. Can. J. Forest Res. 29, 1627–1632.

23.	Bevan, M. (1984) Binary Agrobacterium vectors for plan transformation. Nucleic Acids Res. 12, 8711–8721.

24.	Ecker, P., Roshal, S., Schell, J., and Willmitzer, L. (1986) Isolation and characterization of a light-inducible, organ-specific gene from potato and the analysis of its expression after tagging and transfer in tobacco and potato shoots. Mol. Gen. Genet. 199, 216–244.

25.	Vancanneyt, G., Schmidt, R., O’Connor-Sánchez, A., Willmitzer, L., and Rocha-Rosa, M. (1990) Construction of an intron-containing marker gene: splicing of the intron in transgenic plants and its use in monitoring early events in Agrobacterium-mediated plant transformation. Mol. Gen. Genet. 220, 245–250.

26.	Murashige, T. and Skoog, F. (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15, 473–497.

27.	Gamborg, O.L. (1966) Aromatic metabolism in plants. II. Enzymes of the shikimate pathway in suspension cultures of plant cells. Can. J. Biochem. 44, 791–799.

28.	Schenk, R.U. and Hildebrandt, A.C. (1972) Medium and techniques for induction and growth of monocotyledonous and dicotyledonous plant cell cultures. Can. J. Bot. 50, 199–204.

29.	An, G., Ebert, P.R., Mitra, A., and Ha, S.B. (1988) Binary vectors. In: Plant Molecular Biology Manual (Gelvin, S.B., Schilperoort, R.A., Verma, D. PS., eds.), Kluwer Academic Publishers, Dordrecht, Netherlands, pp. 1–19.

30.	Wiley, J. and Sons (eds.) (2000) Current Protocols in Molecular Biology Online, John Wiley and Sons, New York, NY.

31.	Jefferson, R.A. (1987) Assaying chimeric genes in plants: the GUS gene system. Plant. Mol. Biol. Rep. 5, 387–405.

32.	Gelvin, S.B. (2003) Agrobacterium-mediated plant transformation: the biology behind the “gene-jockeying” tool. Microbiol. Mol. Biol. Rev. 67, 16–37.

Comments (Loading...)

Post comment/View all comments