SpringerProtocols: Abstract: Soybean (Glycine max) Transformation Using Mature Cotyledonary Node Explants

Soybean (Glycine max) Transformation Using Mature Cotyledonary Node Explants

By: Paula M. Olhoft, Christopher M. Donovan, David A. Somers

Abstract

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Agrobacterium tumefaciens-mediated transformation of soybeans has been steadily improved since its development in 1988. Soybean transformation is now possible in a range of genotypes from different maturity groups using different explants as sources of regenerable cells, various selectable marker genes and selective agents, and different A. tumefaciens strains. The cotyledonary-node method has been extensively investigated and across a number of laboratories yields on average greater than 1% transformation efficiency (one Southern-positive, independent event per 100 cotyledonary-node explants). Continued improvements in the cotyledonary-node method concomitant with further increases in transformation efficiency will enhance broader adoption of this already productive transformation method for use in crop improvement and functional genomics research efforts.

Book Title: Agrobacterium Protocols

Series: Methods in Molecular Biology | Volume: 343 | Pub. Date: Jun-01-2006 | Page Range: 385-396 | DOI: 10.1385/1-59745-130-4:385

Subject: Plant Sciences

Key Words: Genetic engineering - transgenic - legumes - axillary meristems - hygromycin phosphotransferase - Mendelian inheritance - nuclear transformation

References

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1.	McCabe, D. E., Swain, W. F., Martinell, B. J., and Christou, P. (1988) Stable transformation of soybean (Glycine max) by particle acceleration. Bio/Technology 6, 923–926.

2.	Parrott, W. A., Hoffman, L. M., Hildebrand, D. F., Williams, E. G., and Collins, G. B. (1989) Recovery of primary transformants of soybean. Plant Cell Rep. 7, 615–617.

3.	Finer, J. J. and McMullen, M. D. (1991) Transformation of soybean via particle bombardment of embryogenic suspension culture tissue. In Vitro Cell Devel. Biol. 27P, 175–182.

4.	Ko, T. S., Lee, S., Krasnyanski, S., and Korban, S. S. (2003) Two critical factors are required for efficient transformation of multiple soybean cultivars: Agrobacterium strain and orientation of immature cotyledonary explant. Theor. Appl. Genet. 107, 439–447.

5.	Yan, B., Reddy, M. S. S., Collins, G. B., and Dinkins, R. D. (2000) Agrobacterium tumefaciens-mediated transformation of soybean [Glycine max (L.) Merrill] using immature zygotic cotyledon explants. Plant Cell Rep. 19, 1090–1097.

6.	Ko, T.-S., Sangman, L., Farrand, S. K., and Korban, S. S. (2004) A partially disarmed vir helper plasmid, pKYRT1, in conjunction with 2,4-dichlorophenoxy-acetic acid promotes emergence of regenerable transgenic somatic embryos from immature cotyledons of soybean. Planta 218, 536–541.

7.	Trick, H. N. and Finer, J. J. (1998) Sonication-assisted Agrobacterium-mediated transformation of soybean [Glycine max (L.) Merrill] embryogenic suspension culture tissue. Plant Cell Rep. 17, 482–488.

8.	Martinell, B. J., Julson, L. S., Emler, C. A., et al. (2002) Soybean Agrobacterium transformation method. U.S. Patent Application No. 6384301.

9.	Aragao, F. J. L., Sarokin, L., Vianna, G. R., and Rech, E. L. (2000) Selection of transgenic meristematic cells utilizing a herbicidal molecule results in recovery of fertile transgenic soybean [Glycine max (L.) Merrill] plants at a high frequency. Theor. Appl. Genet. 101, 1–6.

10.	Liu, H.-K., Yang, C., and Wei, Z.-M. (2004) Efficient Agrobacterium tumefaciens-mediated transformation of soybeans using an embryonic tip regeneration system. Plant Cell Rep. 219, 1042–1049.

11.	Hinchee, M. A. W., Connor-Ward, D. V., Newell, C. A., et al. (1988) Production of transgenic soybean plants using Agrobacterium-mediated DNA transfer. Bio/Technology 6, 915–922.

12.	Trick, H. N., Dinkins, R. D., Santarem, E. R., et al. (1997) Recent advances in soybean transformation. Plant Tissue Culture Biotechnol. 3, 9–26.

13.	Olhoft, P. M., Lin, K., Galbraith, J., Nielsen, N. C., and Somers, D. A. (2001) The role of thiol compounds in increasing Agrobacterium-mediated transformation of soybean cotyledonary-node cells. Plant Cell Rep. 20, 731–737.

14.	Olhoft, P. M. and Somers, D. A. (2001) L-cysteine increases Agrobacterium-mediated T-DNA delivery into soybean cotyledonary-node cells. Plant Cell Rep. 20, 706–711.

15.	Olhoft, P. M., Flagel, L. E., Donovan, C. M., and Somers, D. A. (2003) Efficient soybean transformation using hygromycin B selection in the cotyledonary-node method. Planta 216, 723–735.

16.	Zhang, Z., Xing, A., Staswick, P., and Clemente, T. (1999) The use of glufosinate as a selective agent in Agrobacterium-mediated transformation of soybean. Plant Cell Tissue Organ Culture 56, 37–46.

17.	Zeng, P., Vadnais, D. A., Zhang, Z., and Polacco, J. C. (2003) Refined glufosinate selection in Agrobacterium-mediated transformation of soybean [Glycine max (L.) Merrill]. Plant Cell Rep. 22, 478–482.

18.	Olhoft, P. M., Flagel, L. E., and Somers, D. A. (2004) T-DNA locus structure in a large population of soybean plants transformed using the Agrobacterium-mediated cotyledonary-node method. Plant Biotechnol. J. 2, 289–300.

19.	Donaldson, P. A. and Simmonds, D. H. (2000) Susceptibility to Agrobacterium tumefaciens and cotyledonary node transformation in short-season soybean. Plant Cell Rep. 19, 478–484.

20.	Somers, D., Olhoft, P., Makarevitch, I., and Svitashev, S., (2004) Mechanism(s) of transgene locus formation, in Genetic Transformation in Crop Plants (LIANG, G. and SKINNER, D., eds.), Haworth Press, NY, pp. 17–69.

21.	Somers, D. A. and Makarevitch, I. (2004) Transgene integration in plants: poking or patching holes in promiscuous genomes? Curr. Opin. Biotechnol. 15, 126–131.

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