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Canola (Brassica napus L.)
Abstract
High-frequency Agrobacterium tumefaciens-mediated transformation can be obtained in canola by optimizing the preconditioning time of the explant and cocultivation time with A. tumefaciens. A preconditioning time of 72 h and cocultivation of 48 h synergistically increase the transformation efficiency to 25%. In addition, the recovery of transgenic plants can be facilitated by overcoming hyperhydration, which increases the rooting frequency to 100%.
Series: Methods in Molecular Biology  |  Volume: 343  |  Pub. Date: Jun-01-2006  |  Page Range: 257-266  |  DOI: 10.1385/1-59745-130-4:257
Subject:  Plant Sciences
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