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Agrobacterium Transformation of Arabidopsis thaliana Roots: A Quantitative Assay
Abstract
Arabidopsis thaliana has become a major model system for investigating plant molecular, genetic, and biochemical processes. Arabidopsis is highly susceptible to Agrobacterium-mediated transformation using “flower dip” and “vacuum infiltration” protocols. However, Arabidopsis has also become a major system to investigate the mechanism of Agrobacterium-mediated transformation of somatic tissue. Such investigations require a reproducible, quantitative assay system to determine transformation frequency. We describe here an Arabidopsis root transformation protocol that can be used to determine transformation frequencies for wild-type and mutant Agrobacterium strains and for various Arabidopsis wild-type and mutant lines.
Series: Methods in Molecular Biology  |  Volume: 343  |  Pub. Date: Jun-01-2006  |  Page Range: 105-114  |  DOI: 10.1385/1-59745-130-4:105
Subject:  Plant Sciences
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