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4. An Introduction to PCR Techniques
By: Gill Rumsby3
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The polymerase chain reaction (PCR) is a method for the enzymatic amplification of DNA and has revolutionised our approach
to both diagnostics and research, permitting the amplification of small amounts of genetic material, either derived from genomic
(chromosomal DNA) or RNA via reverse transcription to cDNA. This chapter gives a primer of methods for the preparation of
materials for PCR and some advice on the requirements for the PCR itself.
Affiliation(s): (3) EurClinChem Department of Clinical Biochemistry, University College London Hospitals, London, UK
Book Title: Hormone Assays in Biological Fluids
Series: Methods in Molecular Biology | Volume: 324 | Pub. Date: Jan-16-2006 | Page Range: 75-89 | DOI: 10.1385/1-59259-986-9:75
Subject: Biochemistry
Key Words: Polymerase chain reaction (PCR) - primers - DNA extraction - RNA preparation - reverse transcriptase PCR (RT-PCR) - Taq polymerase
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