SpringerProtocols: Abstract: 3. Gas Chromatography-Mass Spectrometry

3. Gas Chromatography-Mass Spectrometry

By: John W. Honour³

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Gas chromatography-mass spectrometry (GC-MS) has been used in hormone assays particularly for steroids in biological fluids. The combination of GC with MS exploits the high-resolving power of gas chromatography to separate closely related molecules, and the ability of the MS to provide precise data for identification and quantification of the separated substances. GC-MS is a very powerful technique for analysis with specificity of hormones in biological fluids. The general principles of GC-MS are described in this chapter along with some examples that illustrate specific applications of hormone analysis.

Affiliation(s): (3) Department of Clinical Biochemistry, University College London Hospital, London, UK

Book Title: Hormone Assays in Biological Fluids

Series: Methods in Molecular Biology | Volume: 324 | Pub. Date: Jan-16-2006 | Page Range: 53-74 | DOI: 10.1385/1-59259-986-9:53

Subject: Biochemistry

Key Words: Gas chromatography - mass spectrometry - hydroxyl groups - oxo groups - carboxyl groups - steroids - thyroid hormones - melatonin - diabetes

References

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