SpringerProtocols: Abstract: Yeast Transformation by the LiAc/SS Carrier DNA/PEG Method

Yeast Transformation by the LiAc/SS Carrier DNA/PEG Method

By: R. Daniel Gietz², Robin A. Woods³

Abstract

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The technique for the transformation of Saccharomyces cerevisiae using the LiAc/SS Carrier DNA/PEG method is described. We describe a rapid method, for use when large numbers of transformants are not necessary. A high-efficiency method for the generation of large numbers of transformants is also given. A method for the transformation of plasmid libraries, which includes yeast two-hybrid applications, also is listed to aid the reader in generating transformants to effectively cover the library complexity. Finally, a protocol for transformation using a 96-well format is included for transformation applications that require it.

Affiliation(s): (2) Department of Biochemistry and Medical Genmetics, University of Manitoba, Winnipeg, Manitoba, Canada
(3) Department of Biology, University of Winnipeg, Winnipeg, Manitoba, Canada

Book Title: Yeast Protocol

Series: Methods in Molecular Biology | Volume: 313 | Pub. Date: Sep-01-2005 | Page Range: 107-120 | DOI: 10.1385/1-59259-958-3:107

Subject: Microbiology

References

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