SpringerProtocols: Abstract: Yeast Fluorescence Microscopy

Yeast Fluorescence Microscopy

By: Jiří Hašek²

Abstract

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Fluorescence microscopy is the essential technique for investigation of the intracellular distribution of macromolecules and various organelles also in yeast cells. In this chapter, detailed practical procedures for fluorescence microscopic observations developed or adopted in our laboratory are described. These include labeling of the cell wall and chitin, F-actin structures, nuclear and mitochondrial DNA, and two different procedures for investigation of yeast cells by immunofluorescence. In addition, our experience with multicolor labeling experiments is introduced and discussed.

Affiliation(s): (2) Laboratory of Cell Production, Institute of Microbiology, Academy of Sciences of the Czech Republic, The Czech Republic

Book Title: Yeast Protocol

Series: Methods in Molecular Biology | Volume: 313 | Pub. Date: Sep-01-2005 | Page Range: 85-96 | DOI: 10.1385/1-59259-958-3:085

Subject: Microbiology

References

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