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Analysis of Yeast Lipids
Abstract
The precise quantitative determination of the different lipid classes in mutant cells is key to understand the possible role of the respective gene product in lipid homeostasis. In this chapter, we describe methods based on thin-layer chromatography that are employed routinely to determine the level and relative composition of the major lipid classes from yeast.
Affiliation(s): (2) Division of Biochemistry, University of Fribourg, Fribourg, Switzerland
(3) Institute of Biochemistry, Graz University of Technology, Austria
Book Title: Yeast Protocol
Series: Methods in Molecular Biology  |  Volume: 313  |  Pub. Date: Sep-01-2005  |  Page Range: 75-84  |  DOI: 10.1385/1-59259-958-3:075
Subject:  Microbiology
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