SpringerProtocols: Abstract: Natural Product Isolation: An Overview

Natural Product Isolation: An Overview

By: Satyajit D. Sarker⁴, Zahid Latif⁵, Alexander I. Gray⁶

Abstract

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There has been a remarkable resurgence of interest in natural product research over the last decade or so. With the outstanding developments in the areas of separation science, spectroscopic techniques, and microplate-based ultrasensitive in vitro assays, natural product research is enjoying renewed attention for providing novel and interesting chemical scaffolds. The various available hyphenated techniques, e.g., GC-MS, LC-PDA, LC-MS, LC-FTIR, LC-NMR, LC-NMR-MS, CE-MS, have made possible the preisolation analyses of crude extracts or fractions from different natural sources, isolation and on-line detection of natural products, chemotaxonomic studies, chemical finger printing, quality control of herbal products, dereplication of natural products, and metabolomic studies. While different chapters in this book are devoted to a number of specific aspects of natural product isolation protocols, this chapter presents, with practical examples, a general overview of the processes involved in natural product research, starting from extraction to determination of the structures of purified products and their biological activity.

Affiliation(s): (4) Pharmaceutical Biotechnology Research Group, School of Biomedical Sciences, University of Ulster at Coleraine, Coleraine, Northern Ireland, UK
(5) Molecular Nature Limited, Plas Gogerddan, Aberystwyth, Wales, UK
(6) Phytochemistry Research Laboratories, Department of Pharmaceutical Sciences, University of Strathcyclde, Glasgow, Scotland, UK

Book Title: Natural Products Isolation

Series: Methods in Biotechnology | Volume: 20 | Pub. Date: Sep-19-2005 | Page Range: 1-25 | DOI: 10.1385/1-59259-955-9:1

Subject: Biochemistry

Key Words: Natural products - secondary metabolite - extraction - isolation - bioassay

References

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