SpringerProtocols: Abstract: Methods for Measuring TGF-β Using Antibodies, Cells, and Mice

Methods for Measuring TGF-β Using Antibodies, Cells, and Mice

By: Vladimir Jurukovski⁴, Branka Dabovic⁵, Vesna Todorovic⁵, Yan Chen⁵, Daniel B. Rifkin⁵

Abstract

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The transforming growth factor (TGF)-βs are essential in pre- and postnatal development, differentiation and morphogenesis of higher organisms. Quantitation of the levels of TGF-β synthesis, secretion, and activation are crucial for grasping the mechanisms that control these events and ultimately control TGF-β action. Rather than presenting a single method, we describe several methods for measuring active TGF-β in different experimental situations. This is possible as a result of advances in transgenic mice technology that allow in vivo TGF-β measurements in addition to the more established in vitro approaches.

Affiliation(s): (4) Department of Biochemistry and Cell Biology, SUNY at Stony Brook, Stony Brook, NY
(5) Departments of Cell Biology and Medicine, New York University School of Medicine, New York, NY

Book Title: Fibrosis Research: Methods and Protocols

Series: Methods in Molecular Medicine | Volume: 117 | Pub. Date: Aug-08-2005 | Page Range: 161-175 | DOI: 10.1385/1-59259-940-0:161

Subject: Molecular Medicine

Key Words: TGF-β - measurement - mink lung epithelial assay - luciferase - GFP - ELISA - immunohistochemistry - Smad - transgenic mice

References

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