SpringerProtocols: Abstract: Isolation and Culture of Skin Fibroblasts

Isolation and Culture of Skin Fibroblasts

By: Laure Rittié⁴, Gary J. Fisher⁴

Abstract

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Dermal fibroblasts synthesize and organize skin connective tissue (dermis). Fibroblast cultures provide a powerful tool for investigating both the molecular mechanisms that regulate skin connective tissue production and the pathophysiology of fibrotic skin diseases. In this chapter, we describe detailed procedures for establishing and maintaining primary cultures of adult human fibroblasts. We also provide protocols for constructing three-dimensional dermal and skin equivalent culture models.

Affiliation(s): (4) Department of Dermatology, University of Michigan, Ann Arbor, MI

Book Title: Fibrosis Research: Methods and Protocols

Series: Methods in Molecular Medicine | Volume: 117 | Pub. Date: Aug-08-2005 | Page Range: 83-98 | DOI: 10.1385/1-59259-940-0:083

Subject: Molecular Medicine

Key Words: Fibrosis - fibroblast - collagen - organ culture - cell culture - organotyptic culture - skin equivalent

References

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