SpringerProtocols: Abstract: Isolation, Propagation, and Titration of Human Immunodeficiency Virus Type 1 From Peripheral Blood of Infected Individuals

Contents of this article

Browse by Subject

Upload a Protocol

Upload your own protocols for personal use.

Protocol Alert

Receive e-mail notifications about new content on SpringerProtocols.

Video Protocols

View our video protocols.

Call for video protocols.

Comments

Read comments by other users and add your own.

Favorites

Save your favorite protocols to your My Protocols area.

RSS

RSS Feeds

Inside SpringerProtocols

Useful Tools

Isolation, Propagation, and Titration of Human Immunodeficiency Virus Type 1 From Peripheral Blood of Infected Individuals

By: Hanneke Schuitemaker², Neeltje A. Kootstra²

Abstract

Full Text

Download PDF (229K)

HIV-1 can be isolated from peripheral blood mononuclear cells and is easily propagated on primary cells in vitro. Here we describe the method for bulk isolation of the HIV-1 quasispecies and a limiting dilution virus isolation protocol by which single coexisting clones can be obtained. In addition, methods for propagation and titration of HIV-1 are provided.

Affiliation(s): (2) Department of Clinical Viro-Immunology Sanquin Research at CLB and Landsteiner Laboratory at the Academical Medical Center Amsterdam, The Netherlands

Book Title: Human Retrovirus Protocols: Virology and Molecular Biology

Series: Methods in Molecular Biology | Volume: 304 | Pub. Date: May-17-2005 | Page Range: 17-24 | DOI: 10.1385/1-59259-907-9:017

Subject: Infectious Diseases

Key Words: HIV isolation - bulk isolate - HIV variant - TCID50 - stock preparation

Comments (Loading...)

Post comment/View all comments

1.	Popovic, M., Sarngadharan, M. G., Read, E., and Gallo, R. C. (1984) Detection, isolation and continuous production of cytopathic retroviruses (HTLV-III) from patients with AIDS and pre-AIDS. Science 224, 497.

2.	Sawyer, L. S. W., Wrin, M. T., Crawford-Mikza, L., Potts, B., Wu, Y., Weber, P. A., et al. (1994) Neutralization sensitivity of human immunodeficiency virus type 1 is determined in part by the cell in which the virus is propagated. J. Virol. 68, 1342–1349.

3.	De Roda Husman, A. M., Koot, M., Cornelissen, M., Brouwer, M., Broersen, S. M., Bakker, M., et al. (1997) Association between CCR5 genotype and the clinical course of HIV-1 infection. Ann. Intern. Med. 127, 882–890.

4.	Schuitemaker, H., Koot, M., Kootstra, N. A., Dercksen, M. W., De Goede, R. E. Y., Van Steenwijk, R. P., et al. (1992) Biological phenotype of human immunodeficiency virus type 1 clones at different stages of infection: progression of disease is associated with a shift from monocytotropic to T-cell-tropic virus populations. J. Virol. 66, 1354–1360.