SpringerProtocols: Abstract: Peptide-Conjugated Quantum Dots: Imaging the Angiotensin Type 1 Receptor in Living Cells

Peptide-Conjugated Quantum Dots: Imaging the Angiotensin Type 1 Receptor in Living Cells

By: Ian D. Tomlinson², John N. Mason³, Randy D. Blakely³, Sandra J. Rosenthal²

Abstract

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Peptide-quantum dot conjugates have been prepared by attaching angiotensin II (Ang II) to cadmium selenide/zinc sulfide core-shell nanocrystals using an 1-[3-(Dimethyamino)propyl]-3-ethylcarbo diimide hydrochloride (EDC) coupling. These conjugates have been used to image angiotensin I-expressing Chinese hamster ovary (CHO) cells in vitro. When CHO cells were incubated with Ang II before incubating with Ang II-conjugated quantum dots, we were able to block the binding of the dots. The Ang II-quantum dot conjugates did not bind to parental cells and showed similar staining patterns when compared with a commercially available Ang II Alexa 488™ conjugate.

Affiliation(s): (2) Department of Chemistry, Vanderbilt University, Nashville, TN
(3) Department of Pharmacology, Vanderbilt University School of Medicine, Vanderbilt University, Nashville, TN

Book Title: NanoBiotechnology Protocols

Series: Methods in Molecular Biology | Volume: 303 | Pub. Date: Apr-27-2005 | Page Range: 51-60 | DOI: 10.1385/1-59259-901-X:051

Subject: Biotechnology

Key Words: Nanocrystals - quantum dot - angiotensin II - peptide - fluorescence - Chinese hamster ovary cells - Alexa 488™

References

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