Springer Protocols: Abstract: Prion Propagation in Cell Culture

Prion Propagation in Cell Culture

By: Sylvain Lehmann²

Abstract

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During the past two decades, considerable efforts have been made to set up cellular cultures supporting the replication of prions, the infectious agents responsible of transmissible spongiform encephalopathies. As a matter of fact, prion-infected cell lines are very valuable to investigate the cell biology of both the normal and the pathological isoform of the prion protein or to develop and screen new therapeutics. In this chapter, we present a detailed protocol for the generation of prion-infected cells. We also give step-by-step procedures to test the biochemical properties (mainly protease resistance and insolubility) of abnormal PrP molecules, which detection represents a biochemical marker of prion propagation.

Affiliation(s): (2) Institut de Gtique Humaine du CNRS Montpellier, France and Laboratoire de Biochimie, Hôpital St. Eloi, Montpellier, France

Book Title: Amyloid Proteins: Methods and Protocols

Series: Methods in Molecular Biology | Volume: 299 | Pub. Date: Dec-28-2004 | Page Range: 227-234 | DOI: 10.1385/1-59259-874-9:227

Subject: Protein Science

Key Words: Prion - scrapie - PrP - transmissible spongiform encephalopathies - Creutzfeldt-Jakob disease - protease resistance - cell culture - murine neuroblastoma cell line N2a

References

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