SpringerProtocols: Abstract: Murine Cerebrovascular Cells as a Cell Culture Model for Cerebral Amyloid Angiopathy: Isolation of Smooth Muscle and Endothelial Cells From Mouse Brain

Murine Cerebrovascular Cells as a Cell Culture Model for Cerebral Amyloid Angiopathy: Isolation of Smooth Muscle and Endothelial Cells From Mouse Brain

By: Sonia S. Jung³, Efrat Levy²

Abstract

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The use of murine cerebrovascular cells, that is, endothelial and smooth muscle cells, has not been widely employed as a cell culture model for the investigation of cellular mechanisms involved in cerebral amyloid angiopathy (CAA). Difficulties in isolation and propagation of murine cerebrovascular cells and insufficient yields for molecular and cell culture studies have deterred investigators from using mice as a source for cerebrovascular cells in culture. To date, most of the literature has described isolation of smooth muscle cells or endothelial cells from human, canine, rat, guinea pig, or other large animals.

Affiliation(s): (2) Departments of Psychiatry and Pharmacology, New York University School of Medicine, New York, NY
(3) Center for Dementia Research, Nathan S. Kline Institute for Psychiatric Research, Orangeburg, NY

Book Title: Amyloid Proteins: Methods and Protocols

Series: Methods in Molecular Biology | Volume: 299 | Pub. Date: Dec-28-2004 | Page Range: 211-219 | DOI: 10.1385/1-59259-874-9:211

Subject: Protein Science

Key Words: Cerebral blood vessel - microvessel - cerebrovascular cell - smooth muscle cell - endothelial cell - primary cells - cystatin C - amyloid - α-actin - von Willebrand factor - factor VIII-related antigen

References

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