Quasielastic Light Scattering for Protein Assembly Studies
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Quasielastic light scattering (QLS) spectroscopy is an optical method for the determination of diffusion coefficients of particles
in solution. In this chapter, we discuss the principles and practice of QLS with respect to protein assembly reactions. Particles
undergoing Brownian motion produce fluctuations in scattered light intensity. We describe how the temporal correlation function
of these fluctuations can be measured and how this correlation function provides information about the distribution of diffusion
coefficients of the particles in solution. We discuss the intricacies of deconvolution of the correlation function and the
assumptions incorporated into data analysis procedures. We explain how the Stokes-Einstein relationship can be used to convert
distributions of diffusion coefficients into distributions of particle size. Noninvasive observation of the temporal evolution
of particles sizes provides a powerful tool for studying protein aggregation and self-assembly. We use examples from studies
of A_ fibrillogenesis to illustrate QLS application for understanding the molecular mechanisms of the nucleation and growth
of amyloid fibrils.
Affiliation(s): (2) Department of Physics and Center for Materials Science and Engineering, Massachusetts Institute of Technology, Cambridge, MA
(3) Center for Neurologic Diseases, Brigham and Women’s Hospital and Department of Neurology, Harvard Medical School, Boston, MA
(3) Center for Neurologic Diseases, Brigham and Women’s Hospital and Department of Neurology, Harvard Medical School, Boston, MA
Book Title: Amyloid Proteins: Methods and Protocols
Series: Methods in Molecular Biology | Volume: 299 | Pub. Date: Dec-28-2004 | Page Range: 153-174 | DOI: 10.1385/1-59259-874-9:153
Subject: Protein Science
Key Words: Dynamic light scattering - diffusion - size distribution - self-assembly - aggregation - Alzheimer%s disease, amyloid - fibrillogenesis
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