SpringerProtocols: Abstract: 13. Immunogold Probes in Light Microscopy

13. Immunogold Probes in Light Microscopy

By: David Hughes²

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Since their introduction 60 yr ago, immunolabeling procures for use in light microscopy have become increasingly sophisticated, with greater levels of sensitivity and versatility. This chapter reviews the range of immunocytochemical procedures now available, discussing the attributes of each method that must be considered when choosing the most appropriate approach for the tissue target concerned. The immunogold silver staining technique is described in detail because the method has many advantages over rival labeling procedures, including its dual applicability at the light and electron microscope levels.

Affiliation(s): (2) Nuffield Department of Surgery, John Radcliffe Hospital, Oxford, UK

Book Title: Immunochemical Protocols: Third Edition

Series: Methods in Molecular Biology | Volume: 295 | Pub. Date: Dec-13-2004 | Page Range: 173-192 | DOI: 10.1385/1-59259-873-0:173

Subject: Immunology

Key Words: Immunocytochemistry - immunogold - silver enhancement - immunofluorescence - immunoenzyme - avidin biotin - igss - pap - apaap - epipolarization

References

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