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8. Conjugation of Antibodies to Alkaline Phosphatase
By: G. Brian Wisdom2
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Alkaline phosphatase is coupled to immunoglobin G antibody in a one-step procedure using the homobifunctional reagent glutaraldehyde,
which reacts with amino groups in the two proteins. The procedure is simple to perform and requires minimal equipment.
Affiliation(s): (2) School of Biology and Biochemistry, The Queens University, Belfast, Northern Ireland, UK
Book Title: Immunochemical Protocols: Third Edition
Series: Methods in Molecular Biology | Volume: 295 | Pub. Date: Dec-13-2004 | Page Range: 123-126 | DOI: 10.1385/1-59259-873-0:123
Subject: Immunology
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