SpringerProtocols: Abstract: 6. Phage Display Vectors for the In Vitro Generation of Human Antibody Fragments

6. Phage Display Vectors for the In Vitro Generation of Human Antibody Fragments

By: Michael Hust², Stefan Dübel³

Abstract

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A major source of human antibodies are phage display libraries, which are constructed from various genetic sources. Antibodies are expressed as scFV and Fab antibody fragments using various vector systems. This review offers a comprehensive overview of M13 phage display antibody vectors and discusses their applications.

Affiliation(s): (2) Department of Biotechnology, Institute of Biochemistry and Biotechnology, Carolo-Wilhelmina Technical University, Braunschweig, Germany
(3) Professor of Biotechnology, Institute of Biochemistry and Biotechnology, Carolo-Wilhelmina Technical University, Braunschweig, Germany

Book Title: Immunochemical Protocols: Third Edition

Series: Methods in Molecular Biology | Volume: 295 | Pub. Date: Dec-13-2004 | Page Range: 71-96 | DOI: 10.1385/1-59259-873-0:071

Subject: Immunology

Key Words: Antibody (Ab) - fragment antigen binding (Fab) - fragment variable (Fv) - single-chain Fv (scFv) - variable part of - heavy chain - variable part of light chain - wild type

References

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