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5. Growing Hybridomas
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Hybridomas can be grown in a number of ways to produce stocks of monoclonal antibodies. Smaller volumes may be produced in
static flask culture, but if larger amounts are required, a bioreactor may be used.
Affiliation(s): (2) Head of Immunology, Moredun Research Institute, Bush Loan, Edinburgh, Scotland
(3) Immunology Group, Moredun Research Institute, Bush Loan, Edinburgh, Scotlan
(3) Immunology Group, Moredun Research Institute, Bush Loan, Edinburgh, Scotlan
Book Title: Immunochemical Protocols: Third Edition
Series: Methods in Molecular Biology | Volume: 295 | Pub. Date: Dec-13-2004 | Page Range: 55-70 | DOI: 10.1385/1-59259-873-0:055
Subject: Immunology
Key Words: Hybridoma - bioreactor - flask culture - monoclonal - antibodies - culture medium - cryopreservation
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