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4. Making Hybridomas
By: Robert Burns2
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Hybridomas are derived from the fusion of spleen and myeloma cells and produce monoclonal antibodies. Each hybridoma cell
line produces an antibody with a unique specificity allowing the production of highly defined reagents that can be used in
many branches of immunochemistry.
Book Title: Immunochemical Protocols: Third Edition
Series: Methods in Molecular Biology | Volume: 295 | Pub. Date: Dec-13-2004 | Page Range: 41-53 | DOI: 10.1385/1-59259-873-0:041
Subject: Immunology
Key Words: Monoclonal antibodies - cell fusion - hybridoma - immunization - cell culture - myeloma - splenocytes
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