SpringerProtocols: Abstract: 2. Synthetic Peptides as Antigens for Antibody Production

2. Synthetic Peptides as Antigens for Antibody Production

By: David C. Hancock², Nicola J. OReilly³

Abstract

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The use of synthetic peptide immunogens as a means to generate specific immunological reagents for a variety of purposes has increased markedly in recent years. In this chapter, we outline some of the salient factors to be considered when designing peptide immunogens and describe basic methodologies for the conjugation of short synthetic peptides to immunogenic carrier proteins.

Affiliation(s): (2) Signal Transduction Laboratory, Cancer Research UK, London Research Institute Laboratories, London, UK
(3) Cancer Research UK, London Research Institute Laboratories, London, UK

Book Title: Immunochemical Protocols: Third Edition

Series: Methods in Molecular Biology | Volume: 295 | Pub. Date: Dec-13-2004 | Page Range: 13-25 | DOI: 10.1385/1-59259-873-0:013

Subject: Immunology

Key Words: Synthetic - peptide - antibody - antigen - polyclonal - immunogen - carrier - conjugation

References

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