Springer Protocols: Abstract: 7. Adenoviral Transduction of Dendritic Cells

7. Adenoviral Transduction of Dendritic Cells

By: Rienk Offringa³, Kitty Kwappenberg³, Martijn Rabelink⁴, Delphine Rea⁴, Rob Hoeben⁴

Abstract

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Recombinant adenoviral (rAd) vectors are highly suitable for efficient genetic modification of dendritic cells (DC). In certain cases, the high immunogenicity of rAd may be a disadvantage This chapter describes the essential aspects of optimal rAd-mediated gene transfer into DC, discusses the consequences of the immunogenicity of rAd vectors, and provides suggestions to minimize these complications.

Affiliation(s): (3) Department of Immunohematology and Blood Transfusion, Leiden University Medical Center, Leiden, The Netherlands
(4) Department of Molecular Cell Biology, Leiden University Medical Center, Leiden, The Netherlands

Book Title: Adoptive Immunotherapy: Methods and Protocols

Series: Methods in Molecular Medicine | Volume: 109 | Pub. Date: Nov-30-2004 | Page Range: 83-95 | DOI: 10.1385/1-59259-862-5:083

Subject: Immunology

Key Words: Dendritic cells - recombinant adenovirus - genetic transduction - monocytes

References

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