Springer Protocols: Abstract: 6. Generation of Clinical-Grade Monocyte-Derived Dendritic Cells Using the CliniMACS System

6. Generation of Clinical-Grade Monocyte-Derived Dendritic Cells Using the CliniMACS System

By: Thomas Putz³, Hubert Gander³, Reinhold Ramoner³, Claudia Zelle-Rieser³, Andrea Rahm³, Walter Nussbaumer⁴, Georg Bartsch⁴, Lorenz Höltl⁴, Martin Thurnher⁴

Abstract

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We describe the generation of monocyte-derived dendritic cells (MoDC) from leukapheresis products using the CliniMACS system from Miltenyi Biotec. In a clinical setting, the method turned out to be feasible for the generation of clinical-grade MoDC from patients with metastatic renal-cell carcinoma. MoDC generated with this system exhibited a fully mature phenotype as well as high migratory and T-cell stimulatory capacity.

Affiliation(s): (3) Department of Urology, University of Innsbruck, Innsbruck, Austria
(4) Central Institute of Blood Transfusion, University of Innsbruck, Innsbruck, Austria

Book Title: Adoptive Immunotherapy: Methods and Protocols

Series: Methods in Molecular Medicine | Volume: 109 | Pub. Date: Nov-30-2004 | Page Range: 71-81 | DOI: 10.1385/1-59259-862-5:071

Subject: Immunology

Key Words: Monocyte-derived dendritic cells - immunomagnetic isolation - leukapheresis - purity - CD14 - CD83 - CCR7 - migration - proliferation

References

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