Springer Protocols: Abstract: 5. Isolation and Generation of Clinical-Grade Dendritic Cells Using the CliniMACS System

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5. Isolation and Generation of Clinical-Grade Dendritic Cells Using the CliniMACS System

By: John D. M. Campbell³, Christoph Piechaczek⁴, Gregor Winkels⁴, Edith Schwamborn⁴, Daniela Micheli⁴, Sonja Hennemann⁴, Jürgen Schmitz⁴

Abstract

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Dendritic cells (DC) can either be generated from progenitors such as stem cells or CD 14+ monocytes, or isolated directly from the blood. Blood-derived DC are present as at least two distinct populations-myeloid and plasmacytoid DC. Here we describe methods for the clinical grade isolation of blood DC and DC precursors using the CliniMACS™. We describe the isolation of ultra-pure monocytes in order to generate large numbers of monocyte-derived DC, and also new methods for the direct isolation of blood DC. Isolation of blood DC in large numbers means that natural DC with different properties can be investigated for their clinical function for the first time.

Affiliation(s): (3) Miltenyi Biotec GmbH, Bisley, Surrey, UK
(4) Miltenyi Biotec GmbH, Bergisch Gladbach, Germany

Book Title: Adoptive Immunotherapy: Methods and Protocols

Series: Methods in Molecular Medicine | Volume: 109 | Pub. Date: Nov-30-2004 | Page Range: 55-70 | DOI: 10.1385/1-59259-862-5:055

Subject: Immunology

Key Words: Dendritic cells - myeloid DC - plasmacytoid DC - magnetic separation - CliniMACS

References

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