SpringerProtocols: Abstract: Assessment of RET/PTC Oncogene Activation in Thyroid Nodules Utilizing Laser Microdissection Followed by Nested RT-PCR

Assessment of RET/PTC Oncogene Activation in Thyroid Nodules Utilizing Laser Microdissection Followed by Nested RT-PCR

By: Giovanni Tallini², Guilherme Brandao²

Abstract

Full Text

Download PDF (343K)

Single palpable nodules of the thyroid gland are common in clinical practice; the majority of such lesions are benign. However, noninvasive thyroid nodules that exhibit borderline morphological signs of papillary cancer represent a diagnostic challenge. Rearrangements of the RET oncogene have been proposed as a marker for papillary thyroid cancer. In this chapter, methods for the analysis of the RET oncogene in laser microdissected papillary thyroid cancer tissue are described.

Affiliation(s): (2) Division of Pathology, Yale University School of Medicine, New Haven, CT

Book Title: Laser Capture Microdissection: Methods and Protocols

Series: Methods in Molecular Biology | Volume: 293 | Pub. Date: Dec-23-2004 | Page Range: 103-112 | DOI: 10.1385/1-59259-853-6:103

Subject: Imaging/Radiology

Key Words: Oncogene - RNA extraction - thyroid neoplasm

References

Download References

1.	Vande J.B. Gaston E.A.,and Dawber T.R.(1968) The significance of nontoxic nodules: final report of a 15-year study of the incidence of thyroid malignancy.Ann.Intern.Med. 69, 537–540.

2.	Mazzaferri E. L. (1992) Thyroid cancer in thyroid nodules: finding a needle in the haystack. Am. J. Med. 93, 359–362. <Occurrence Type="DOI"><Handle>10.1016/0002-9343(92)90163-6</Handle></Occurrence>

3.	Rosai, J. (1996) Thyroid gland, in Ackerman’s Surgical Pathology, Mosby, St. Louis, MO, pp. 493–567.

4.	Andreoli, T.E. Carpenter, C.C.J. Bennett, J.C.and Plum, F.(1997) The thyroid gland, in Cecil Essentials of Medicine, W.B.Saunders, Philadelphia, pp.487–496.

5.	Rosai, J., Carcangiu M. L., and Delellis, R. A. (1992) Tumors of the thyroid gland, in Atlas of Tumor Pathology, Third Series, Fascicle 5, Armed Forces Institute of Pathology, Washington, DC.

6.	Grieco, M., Santoro, M., Berlingieri, M. T., et al. (1990) PTC is a novel rearranged form of the ret proto-oncogene and is frequently detected in vivo in human thyroid papillary carcinomas.Cell 60, 557–563. <Occurrence Type="DOI"><Handle>10.1016/0092-8674(90)90659-3</Handle></Occurrence>

7.	Santoro, M., Sabino, N., Ishizaka, Y., et al. (1993) Involvement of RET oncogene in human tumors: specificity of RET activation to thyroid tumors. Br. J. Cancer 68,460–464.

8.	Santoro, M., Dathan, N. A., Berlingeri, M. T., et al. (1994) Molecular characteristics or RET/PTC3: a novel rearranged version of the RET protooncogene in a human thyroid papillary carcinoma. Oncogene 9, 509–516.

9.	Fusco, A., Chiapetta, G., Hui, P., et al. (2002) Assessment of RET/PTC oncogene activation and clonality in thyroid nodules with incomplete morphological evidence of papillary carcinoma-A search for the early precursor of papillary cancer.Am.J.Path. 160, 2157–2167.

10.	Greene, F.L., Page, D.L., Fleming, I.D., et al. (2002) AJCC Cancer Staging Manual, 6th edition. Springer-Verlag, New York, pp.77–87.

11.	Williams, E. D. (2000) Guest editorial: two proposals regarding the terminology of thyroid tumors. Internat. J. Surg. Pathol. 8,181–183.

12.	Izzo, P., Costanzo, P., Lupo, A., et al. (1987) A new human species of aldolase A mRNA from fibroblasts. Eur. J. Biochem. 164, 9–13.

13.	Viglietto, G., Chiappetta, G., Fukunaga, et al. (1995) RET/PTC oncogene activation is an early event in thyroid carcinogenesis. Oncogene 11,1207–1210.

14.	Tallini, G., Santoro, M., Helie, M., et al. (1998) RET/PTC oncogene activation defines a subset of papillary thyroid carcinomas lacking evidence of progression to poorly differentiated or undifferentiated tumor phenotypes. Clin. Cancer Res. 4, 287–294.

15.	Huang, L. E., Luzzi, V., Ehrig, T., Holtschlag, V., and Watson, M. A. (2002) Optimized tissue processing and staining for laser capture microdissection and nucleic acid retrieval. Methods Enzymol. 356, 49–62.

16.	Fend, F., Specht, K., Kremer, M., and Quintanilla-Martinez, L. (2002) Laser capture microdissection in pathology. Methods Enzymol. 356, 196–206.

17.	Sheehan, D. C. and Hrapchak, B. B. (1980) Theory and Practice of Histotechnology, Battle Press, Columbus, OH, 2nd edition 1980, p. 151.

18.	Goldsworthy, S. M., Stockton, P. S., Trempus, C. S., Foley, J. F., and Maronpot, R. R. (1999) Effects of fixation on RNA extraction and amplification from laser capture microdissected tissue. Mol. Carcinog. 25, 86–91. <Occurrence Type="DOI"><Handle>10.1002/(SICI)1098-2744(199906)25:2<86::AID-MC2>3.0.CO;2-4</Handle></Occurrence>

Comments (Loading...)

Post comment/View all comments