SpringerProtocols: Abstract: 11. Hematopoietic Development of Human Embryonic Stem Cells in Culture

11. Hematopoietic Development of Human Embryonic Stem Cells in Culture

By: Xinghui Tian³, Dan S. Kaufman³

Abstract

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The isolation of embryonic stem (ES) cells from human preimplantation blastocysts creates an exciting new starting point to analyze the earliest stages of human blood development. This chapter describes two methods to promote hematopoietic differentiation of human ES cells: stromal cell coculture and embryoid body formation. Better understanding of basic human hematopoiesis through the study of human ES cells will likely have future therapeutic benefits.

Affiliation(s): (3) Stem Cell Institute and Department of Medicine, University of Minnesota, Minneapolis, MN

Book Title: Basic Cell Culture Protocols

Series: Methods in Molecular Biology | Volume: 290 | Pub. Date: Oct-15-2004 | Page Range: 149-162 | DOI: 10.1385/1-59259-838-2:149

Subject: Cell Biology

Key Words: Embryonic stem cells - embryoid bodies - hematopoietic differentiation - mouse embryonic fibroblast feeders - stromal coculture

References

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