SpringerProtocols: Abstract: Chemical Methods for Peptide-Oligonucleotide Conjugate Synthesis

Chemical Methods for Peptide-Oligonucleotide Conjugate Synthesis

By: Dmitry A. Stetsenko², Michael J. Gait²

Abstract

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Methods of peptide-oligonucleotide conjugate synthesis are presented that may be useful in the study of cell targeting and delivery of oligonucleotides and their analogs. The first method involves total stepwise solid-phase synthesis on a single support. The second involves preparation of oligonucleotides containing 2′-aldehydes and subsequent chemoselective ligation with functionalized peptides to form hydrazine, oxime, or thiazolidine linkages.

Affiliation(s): (2) Laboratory of Molecular Biology, MRC, Cambridge, UK

Book Title: Oligonucleotide Synthesis

Series: Methods in Molecular Biology | Volume: 288 | Pub. Date: Sep-01-2004 | Page Range: 205-224 | DOI: 10.1385/1-59259-823-4:205

Subject: Biochemistry

Key Words: Oligonucleotide - peptide - conjugate - aldehyde - solid-phase synthesis - chemoselective ligation - hydrazone - hydrazine - oxime - thiazolidine

References

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