SpringerProtocols: Abstract: 13. Synthesis of Oligonucleotide-Peptide and Oligonucleotide-Protein Conjugates

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13. Synthesis of Oligonucleotide-Peptide and Oligonucleotide-Protein Conjugates

By: David R. Corey²

Abstract

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The conjugation of macromolecules offers a rapid and versatile route to improved function. Here, the methods for obtaining disulfide-linked oligonucleotide-peptide and oligonucleotide-protein conjugates are described. These hybrid molecules can be used deliver chemical functionality to specific sequences within deoxyribonucleic acid and ribonucleic acid. The peptides and proteins can also be used to enhance the hybridization properties of the attached oligonucleotides.

Affiliation(s): (2) Departments of Pharmacology and Biochemistry, University of Texas Southwestern Medical Center at Dallas, Dallas, TX

Book Title: Bioconjugation Protocols: Strategies and Methods

Series: Methods in Molecular Biology | Volume: 283 | Pub. Date: Jun-10-2004 | Page Range: 197-206 | DOI: 10.1385/1-59259-813-7:197

Subject: Biochemistry

Key Words: Oligonucleotide - conjugation - disulfide - crosslinking - hybridization - semisynthetic enzyme

References

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1.	Braasch, D. A. and Corey, D. R. (2002) Novel antisense strategies for controlling gene expression. Biochemistry 41, 4503–4510.

2.	Corey, D. R. and Schultz, P. G., (1987) Generation of a hybrid sequence-specific single-stranded deoxyribonuclease. Science 238, 1401–1403.

3.	Zuckermann, R. N., Corey, D. R., and Schultz, P. G. (1988) Site-selective cleavage of RNA by a hybrid enzyme. J. Am. Chem. Soc. 110, 1614–1615.

4.	Corey, D. R., Pei, D., and Schultz, P. G. (1989) The generation of a catalytic oligonucleotide-directed nuclease. Biochemistry 28, 8277–8286.

5.	Corey, D. R., Pei, D., and Schultz, P. G. (1989) The sequence-selective hydrolysis of duplex DNA by an oligonucleotide-directed nuclease. J. Am. Chem. Soc. 111, 8523–8525.

6.	Pei, D., Corey, D. R., and Schultz, P. G. (1990) site-specific cleavage of duplex DNA by a semi-synthetic Nuclease via triple helix formation. Proc. Natl. Acad. Sci. 87, 9858–9862.

7.	Corey, D. R., Munoz-Medellin, D., and Huang, A. (1995) Strand invasion by oligonucleotide-nuclease conjugates. Bioconjugate Chem. 6, 93–100.

8.	Iyer, M., Norton, J. C., and Corey, D. R. (1995) Accelerated hybridization of oligonucleotides to duplex DNA. J. Biol. Chem. 270, 14,712–14,717.

9.	Corey, D. R. (1995) 48,000-fold acceleration of hybridization of chemically modified oligomers to duplex DNA. J. Am. Chem. Soc. 117, 9373–9374.

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