SpringerProtocols: Abstract: 5. Detecting and Quantifying Apoptosis in Tissue Sections

5. Detecting and Quantifying Apoptosis in Tissue Sections

By: Vicki Save², Peter A. Hall³, Philip J. Coates⁴

Abstract

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Various techniques exist for the identification of apoptosis in tissue sections or intact cells. The use of simple morphology, electron microscopy, DNA-end labeling techniques, and immunochemical methods are reviewed, with a particular emphasis on in situ end-labeling. The analysis of apoptotic cells and methods for their quantification are also discussed.

Affiliation(s): (2) Department of Pathology, Addenbrookes Hospital, Cambridge University, Cambridge, UK
(3) Department of Pathology and Cancer Research Centre, Queen’s University, Belfast, Northern Ireland, UK
(4) Division of Pathology and Neurosciences, Ninewells Hospital and Medical School, University of Dundee, Dundee, Scotland, UK

Book Title: Apoptosis Methods and Protocols

Series: Methods in Molecular Biology | Volume: 282 | Pub. Date: May-20-2004 | Page Range: 67-84 | DOI: 10.1385/1-59259-812-9:067

Subject: Cell Biology

Key Words: Apoptosis - ISEL - TUNEL - immunohistochemistry - histology - cytology

References

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