SpringerProtocols: Abstract: 3. Flow Cytometry-Based Methods for Apoptosis Detection in Lymphoid Cells

3. Flow Cytometry-Based Methods for Apoptosis Detection in Lymphoid Cells

By: Owen Williams²

Abstract

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Apoptosis is an active form of cell death that plays a critical role in lymphocyte development, selection and homeostasis. This process is characterized by the activation of biochemical pathways that lead to changes in cellular morphology (including cell shrinkage, membrane blebbing and nuclear condensation), DNA fragmentation, perturbation of mitochondrial membrane function and changes in the plasma membrane. Each of these cellular alterations can be rapidly quantitated in lymphocyte apoptosis using flow cytometry.

Affiliation(s): (2) Molecular Hematology and Cancer Biology Unit, Institute of Child Health, University College London, London, UK

Book Title: Apoptosis Methods and Protocols

Series: Methods in Molecular Biology | Volume: 282 | Pub. Date: May-20-2004 | Page Range: 31-42 | DOI: 10.1385/1-59259-812-9:031

Subject: Cell Biology

Key Words: Lymphocytes - cell death - FACS - DNA fragmentation - mitochondria

References

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