SpringerProtocols: Abstract: Evaluation of Autoimmunity to Transaldolase in Multiple Sclerosis

Evaluation of Autoimmunity to Transaldolase in Multiple Sclerosis

By: Brian Niland², Andras Perl³

Abstract

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Transaldolase is a target of autoimmunity mediated by T cells and antibody (Ab) in patients with multiple sclerosis. Functional T-cell assays, T- and B-cell epitope mapping, and detection of transaldolase-specific antibodies in patients with multiple sclerosis are described. Recombinant transaldolase was produced in a prokaryotic expression vector for use in Western blot analysis of sera of these patients. Overlapping transaldolase peptides 15 amino acids (aa) long were synthesized onto cellulose membranes to map immunodominant B-cell epitopes. Amino acid sequence homologies between viral peptides and immunodominant B-cell epitopes of transaldolase were identified using a computer-based algorithm. Direct assessment of molecular mimicry between transaldolase B-cell epitopes and related viral peptides is also shown. T-cell epitopes are mapped in a T-cell proliferation assay using multiple sclerosis patient and control donor cells. Autoantigen-specific T cells are identified by MHC-peptide tetramer staining using flow cytometry analysis.

Affiliation(s): (2) Departments of Medicine, Microbiology, and Immunology, College of Medicine, Upstate Medical University, State University of New York, Syracuse, NY
(3) Division of Rheumatology, Departments of Medicine and of Microbiology and Immunology, College of Medicine, Upstate Medical University, State University of New York, Syracuse, NY

Book Title: Autoimmunity: Methods and Protocols

Series: Methods in Molecular Medicine | Volume: 102 | Pub. Date: Aug-01-2004 | Page Range: 155-171 | DOI: 10.1385/1-59259-805-6:155

Subject: Immunology

Key Words: Autoimmunity - epitope mapping - MHC-tetramer - molecular mimicry - multiple sclerosis - transaldolase

References

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