SpringerProtocols: Abstract: Solubilization of Aggregates Formed by Expanded Polyglutamine Tract Expression in Cultured Cells

Solubilization of Aggregates Formed by Expanded Polyglutamine Tract Expression in Cultured Cells

By: Noriko Hazeki², Ichiro Kanazawa³

Abstract

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The expression of expanded polyglutamine in mammalian cells causes the formation of aggregates. For elucidation of the biochemical properties of the aggregates, isolation and solubilization of the aggregates are required. This chapter provides useful protocols for the solubilization of polyglutamine aggregates and further applications of protein analysis.

Affiliation(s): (2) Division of Neuroscience, Department of Neurology, CREST, Japan Science and Technology Corporation, Graduate School of Medicine, University of Tokyo, Tokyo, Japan
(3) National Institute of Neuroscience, National Center of Neurology and Psychiatry, and Department of Neurology, CREST, University of Tokyo, Tokyo, Japan

Book Title: Trinucleotide Repeat Protocols

Series: Methods in Molecular Biology | Volume: 277 | Pub. Date: Jun-18-2004 | Page Range: 129-137 | DOI: 10.1385/1-59259-804-8:129

Subject: Cell Biology

Key Words: Polyglutamine - aggregate - formic acid - green fluorescent protein (GFP) - fluorescence-activated cell sorter (FACS) - quantification - Huntington’s disease

References

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